Application: Used for drug toxicity testing.
Unit definition: One unit is the amount of enzyme required to incorporate 1 picomole of TTP in 60 min. at 37°C using polyrA:dT as template.
Assay conditions: 25 mM HEPES-KOH (pH 8.0), 0.5 mM MnCl2, 2.5 mM ß-ME, 10 µg acetylated BSA, 0.01 mM dTTP(pH 7.0), 0.3 µCi (α-³H)dTTP at 88 Ci/mmol, 0.1 M NaCl, 1.6 µg poly (rA)·oligo (dT)50 Incubation is at 37°C for 15 min. in a reaction volume of 15 µl.
Quality Control: The final product exhibits DNA polymerase activity. All preparations are assayed for contaminating endonuclease, 3’- and 5’-exonuclease, nonspecific RNase, and double-stranded DNase activities. This enzyme has endogenous proof reading activity.
Mass Spectroscopic and Biochemical Analyses confirm the presence of human polymerase gamma.
Storage Buffer: 20 mM Tris-HCl, pH 8.0, 50 mM NaCl, 0.05% Triton X-100, 5% (v/v) glycerol, trypsin inhibitor and 10% DMSO.
Storage conditions: Store at -80°C. Avoid repeated freeze-thaw.
1. Gray,H. Wong, T. W.(1992) J. of Biological Chemistry 267, 5835-5841.