RNase I RNase I is a nonspecific ribonuclease that hydrolyzes phosphodiester bond after all four bases.Description:• Only available RNase that cleaves the phosphodiester bond of all four bases.• Degrades RNA to cyclic nucleotide monophosphates leaving a 5'-OH and 2'-, 3'-cyclic monophosphate.• Prefers single-stranded RNA to double-stranded RNA.• Produced from an overexpressing clone in E. coli (2).• Contains no endonuclease or exonuclease activity toward DNA substrates.• No need for boiling prior to use.• Ideal for ribonuclease protection assays.• Useful for mapping or quantitation of RNA by selective cleavage of single-strand regions. Unit Definition: One unit is the amount of enzyme required to degrade 50% of [a-33P] labeled RNA transcript mixed with 2 µg of yeast RNA in 30 min at 37oC as detected by TCA precipitation.Storage Conditions: Store at –20oC.Storage Buffer: 10 mM Tris-HCl (pH 8.0 at 22oC), 200 mM NaCl, 50% glycerol.Quality Control: All preparations are assayed for contaminating exonuclease and nonspecific endonuclease activities.References:1. Meador, J. III and Kennell, D. (1990) Gene 95, 1-7.2. Meador, J. III et. al. (1990) Eur. J. Biochem. 187, 549.